| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.051101 |
| Chromosome: | chromosome 14 |
| Location: | 1059019 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614350 | (1 of 8) PF00169 - PH domain (PH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCACGTGCGGGGCTGCCTGCCTTCAGACTGCGTAGCGGGCGCTTCCCG |
| Internal bar code: | TTTTGGTTCACACAATTTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1983 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGAAACCTTGAGTGCCTC |
| Suggested primer 2: | TGCGTTGGCCTTGTTGATTG |