Insertion junction: CLIP2.051124_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.051124
Chromosome:chromosome 11
Location:1506879
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g468900 FAP404 Flagellar Associated protein 404 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCGGCAACGGCCTCGGCTCGGTTGCCAAGCAGCTGCATTGTGTCAACA
Internal bar code:TATCGAGTTGATTAATCCTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1932
LEAP-Seq percent confirming:94.2857
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTACTATGAGCAGCAGG
Suggested primer 2:ACTGCAAATCGTCCCTCGTT