Insertion junction: CLIP2.051128_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.051128
Chromosome:chromosome 1
Location:4932225
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g034200 (1 of 7) PF00211//PF13416 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_8) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGACAGCGTGCGCCGCATGCGCCAGTCCGCCGCCTCCAAGTCGTCGCT
Internal bar code:GTGTGTCAGTTCTTATGGCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1059
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:AATAGCATGACCGCACCACA
Suggested primer 2:CCAGTGAGGGCAGAAGACTG