Insertion junction: CLIP2.051133_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.051133
Chromosome:chromosome 7
Location:4549544
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344000 (1 of 1) PTHR31988:SF1 - 9-O-ACETYL-N-ACETYLNEURAMINIC ACID DEACETYLASE-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCGGCATACACCACCACCTCTAATCGCCGTGCCTGCCTGCCTGCTTG
Internal bar code:TTTAATCCTTAACCCCGGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1331
LEAP-Seq percent confirming:58.3333
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGACGTGCATACACCA
Suggested primer 2:GACCAAGCTTTGCCCATGTG