| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051143 |
| Chromosome: | chromosome 1 |
| Location: | 7884493 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055440 | CSR8 | Carbohydrate sulfotransferase-related 8; (1 of 1) 2.8.2.30 - [Heparan sulfate]-glucosamine 3-sulfotransferase 3 / Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAGCATGAAGGTGCGTGCGTGTGTGGGGTGTGGGGTGGTGGCGGGTG |
| Internal bar code: | GTTGATTCCTTGCGGACTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3582 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGAACTCCAGGAACCGC |
| Suggested primer 2: | AGGCACCCATCCAGAAATGG |