Insertion junction: CLIP2.051189_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.051189
Chromosome:chromosome 7
Location:1552447
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g324800 SNE6 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGATGAAGTGAAGATGGCACAGAGGAGAACGAGAGAGGAAATGTCAACC
Internal bar code:GGGGTTCAGAAATCGCTTGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3451
LEAP-Seq percent confirming:84.1463
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:GATTGCCACACACCAACCAC
Suggested primer 2:CTGTGCTATTGGCCCCTCAA