| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051195 |
| Chromosome: | chromosome 17 |
| Location: | 4945865 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735450 | SUB14 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTTGCCGCTGCTGCTACTGCCGATGTCACTGTCATCGTCTGCTACAG |
| Internal bar code: | TGGAGGAGGCATCATGTTTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1882 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCACGTCCTCCTTTCCC |
| Suggested primer 2: | CTATGCCGTACACAGGTCCG |