Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.051205 |
Chromosome: | chromosome 5 |
Location: | 2057326 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g236650 | CYG63 | (1 of 5) IPR001054//IPR006189//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // CHASE domain // Nucleotide cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACACGCTTCATGGCTGCTGCAGGCGGCACGGTGGCCACGG |
Internal bar code: | TAACCAGTTAGTGGCGAGCAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 516 |
LEAP-Seq percent confirming: | 60.0 |
LEAP-Seq n confirming: | 30 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCCTTTCCTCCTTACAGCG |
Suggested primer 2: | AAAGCATCTACCAACCCCGG |