| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051207 |
| Chromosome: | chromosome 2 |
| Location: | 7643557 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147700 | (1 of 1) PTHR11271//PTHR11271:SF33 - CHLOROHYDROLASE FAMILY PROTEINS // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCACGGCAGCGGCAGCTGCATCGGAGGCGGCGCGAGCGGCGCGCAGG |
| Internal bar code: | GGCGCGAGAAGCTGGGGAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2411 |
| LEAP-Seq percent confirming: | 92.233 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGAAGCATCGCTACCTAG |
| Suggested primer 2: | GGCTTGACGGCATATGCAAG |