Insertion junction: CLIP2.051224_6

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.051224
Chromosome:chromosome 14
Location:2705437
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g625850 MMP32 Matrix metalloproteinase; (1 of 1) IPR003980//IPR008752 - Histamine H3 receptor // Peptidase M11, gametolysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTTTGCATGACACGCAGATCGACGATGACTGTGACGGCTACGCCGATG
Internal bar code:TGCTCGCTCGAGAGGGCGCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:128
LEAP-Seq percent confirming:56.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:AACTGCAACCCTAGGTTCCG
Suggested primer 2:GACGTCAACACCGCATTCAG