Insertion junction: CLIP2.051227_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.051227
Chromosome:chromosome 9
Location:4606756
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401589 (1 of 2) IPR000104//IPR002893//IPR003072 - Antifreeze protein, type I // Zinc finger, MYND-type // Orphan nuclear receptor, NOR1 type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCGGAGCCGAGCAATGCCCACACCCGCAAGTTGGCGCCAGCAGTGCCG
Internal bar code:ATCCGCATTTATACCGCTAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:3107
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:AACATGTGTCTAGCAGGGCC
Suggested primer 2:CGTTTCCACAAGGGCACAAG