| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051234 |
| Chromosome: | chromosome 16 |
| Location: | 4182439 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670300 | PRP39 | (1 of 1) K13217 - pre-mRNA-processing factor 39 (PRPF39, PRP39); Nuclear pre-mRNA splicing factor and U1 snRNP Component | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGACCATGCTGCTCCCAGCAACCACCAGCACGACACCCCACCGCCGC |
| Internal bar code: | TTATTATGCGAGAGTAAATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2100 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGTGGTTTGTGTCAGCG |
| Suggested primer 2: | ATCTACCCCTTCCTGGCCAT |