| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051237 |
| Chromosome: | chromosome 7 |
| Location: | 1814280 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325730 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTAGCGGCAGCGGAGGAGGAAGAGCTTGCGAGCAGCTGCCAGACGGCG |
| Internal bar code: | CTGCTCTCAACACACTTTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGACTAGGATCCCACGCT |
| Suggested primer 2: | GCAACCTAAGCCCTCAGTGT |