| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051247 |
| Chromosome: | chromosome 3 |
| Location: | 5934718 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188900 | (1 of 2) PF06127 - Protein of unknown function (DUF962) (DUF962) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACGGCCCCGCCTGGGCCTCTCATGCACTGGTGGAGCGCAACCGGCCC |
| Internal bar code: | GTCGCCTCACCGCTGTTCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3841 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAACCCGCCTACATTCCA |
| Suggested primer 2: | TGCTGCGACAGATGATTGGT |