| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051260 |
| Chromosome: | chromosome 10 |
| Location: | 4761093 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGAAGCTTGGCGGGCTGAAGACATACCTGGGAGCGCGATGATGGATG |
| Internal bar code: | TACCGCGAACACTTGGTGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1109 |
| LEAP-Seq percent confirming: | 78.2609 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGGTCTGGGAGAGGAC |
| Suggested primer 2: | TTGCGCACTCCTGCATTAGA |