| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.051287 |
| Chromosome: | chromosome 1 |
| Location: | 6193350 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044100 | AMYB3,AMB3 | Beta-amylase 3; (1 of 3) 3.2.1.2 - Beta-amylase / Saccharogen amylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCAGCCAGGTGCGTTACGCCGCACAAGCGGCGCTGCTGGCAAGCTTC |
| Internal bar code: | GATGAAGCAAAAAGGGAACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1573 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACTGTTCAGGCCAAGTG |
| Suggested primer 2: | TCTTGACACATTGGAGCGCT |