| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051291 |
| Chromosome: | chromosome 12 |
| Location: | 1418557 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488600 | (1 of 1) IPR000104//IPR006059 - Antifreeze protein, type I // Solute-binding family 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTTAAGGCGTACAGTCATGTAGCCAACACCGCTTCCAAAGTGCGCGG |
| Internal bar code: | CTCATTGTAGCGACTTGGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGGGCAAGCTGTGAGTG |
| Suggested primer 2: | TGCAGACTGACGCCAATCAT |