Insertion junction: CLIP2.051291_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.051291
Chromosome:chromosome 12
Location:1418558
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g488600 (1 of 1) IPR000104//IPR006059 - Antifreeze protein, type I // Solute-binding family 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCCATTGTAAAGGCCATGGCACTGTGAAGGAGGGCAGAATGAATACG
Internal bar code:TGTTGGGTCGGGGGTAGGGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3041
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGACTGACGCCAATCAT
Suggested primer 2:TAGTGGGCAAGCTGTGAGTG