| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051302 |
| Chromosome: | chromosome 13 |
| Location: | 4138733 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591250 | EZY20 | (1 of 726) IPR011009 - Protein kinase-like domain; Early zygote expressed protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAACACCAGTCCAACCCCAATCCAACCCCACCCCACCCCACCCACTT |
| Internal bar code: | GCAGCGCTCATTTTATGCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTTGGGTTTCACCTGC |
| Suggested primer 2: | GACCGATATGAGGGGTGCAG |