Insertion junction: CLIP2.051303_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.051303
Chromosome:chromosome 16
Location:4647407
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g667450 TLP2 (1 of 3) PF01167 - Tub family (Tub); Tubby-Like Protein 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGAGCGCAACCTTTGCACGCACCGCTTCGTAAGTTCTTACAGCACCA
Internal bar code:GGTTATGGGTGGACCGATTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3799
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AATGACGGTGGTGCTAGTGG
Suggested primer 2:AAAATTTGTGTACGCCCGCC