| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051311 |
| Chromosome: | chromosome 7 |
| Location: | 654759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317050 | UBQ6 | Ubiquitin; (1 of 2) IPR000626//IPR001841//IPR019956//IPR029071 - Ubiquitin domain // Zinc finger, RING-type // Ubiquitin // Ubiquitin-related domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCATTGCAGTCCAAGGCCCAAATTTGGGTGCAGAGTCCCGAGCCTGC |
| Internal bar code: | GACACTGAAAAAAGTGAGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1749 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGAACACAAACTGGCGCC |
| Suggested primer 2: | GCAGGTTCGTCCTCCGATAG |