| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051315 |
| Chromosome: | chromosome 17 |
| Location: | 4713442 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732900 | (1 of 1) IPR000104//IPR001126 - Antifreeze protein, type I // UmuC domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGCGTTCTTTTGCCAGGTGTGCATTAGCCCGTCGCGGGCCAGGAAG |
| Internal bar code: | GCAAAGGTGGAGAATTCACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1397 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGATGTCCTGGTGCTGCT |
| Suggested primer 2: | GGAACAGGTTTCCGCAACAC |