| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051315 |
| Chromosome: | chromosome 17 |
| Location: | 4713444 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732900 | (1 of 1) IPR000104//IPR001126 - Antifreeze protein, type I // UmuC domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATCGGCATGCAGCACCAGCAGGTCCGACGTGGGCGAAGCACTCGCAG |
| Internal bar code: | AGAGGGATATTCTACGAGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1256 |
| LEAP-Seq percent confirming: | 48.9796 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACAGGTTTCCGCAACAC |
| Suggested primer 2: | GATGATGTCCTGGTGCTGCT |