Insertion junction: CLIP2.051326_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.051326
Chromosome:chromosome 3
Location:1788366
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g153950 TRP12 Transient receptor potential ion channel protein; (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTACAGTGCGCGCGCGGCGAGCAACATGATGCTTGCCGTTTCCTTCTAC
Internal bar code:TAGCCCAGGATACTTTTGTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4986
LEAP-Seq percent confirming:98.7805
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:GCGACGGCTTCAACATCATC
Suggested primer 2:TGCCAATCAACGCTCTGTCT