| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.051335 |
| Chromosome: | chromosome 10 |
| Location: | 5958732 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461250 | NSF1 | (1 of 1) K06027 - vesicle-fusing ATPase (NSF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTCGCCTGTCACGTTGGCGCTGTTCCGCAGGTGACCATGGCGGACT |
| Internal bar code: | CATACTACGTCTCTAGTAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACACATGCACAAACACG |
| Suggested primer 2: | CTCGCCACATCCTGGATCTC |