| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051339 |
| Chromosome: | chromosome 3 |
| Location: | 8005815 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201851 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTGGACCCGCAGCTGCGGCGGCGCCAGTGAGGGGCAGATTATTTTCA |
| Internal bar code: | GATTTCGGAATGATAGTTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3917 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTATCCACAGCCACTG |
| Suggested primer 2: | TGTCCTCATCCGTAGCAGGA |