| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051353 |
| Chromosome: | chromosome 16 |
| Location: | 3303682 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666500 | BBS8 | (1 of 1) K16781 - tetratricopeptide repeat protein 8 (TTC8, BBS8); Bardet Biedl Syndrome-8 associated protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGCGATGGGGGTTGCCGCAGGTTGGTGAGCACACTGACATACCACGA |
| Internal bar code: | TTTTTAATGTTTTACCGACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1580 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGCCCCTTCTTGTAAT |
| Suggested primer 2: | TCAGCCATAAAGAGTGCGCA |