| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051354 |
| Chromosome: | chromosome 12 |
| Location: | 6216212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536000 | ALA2 | (1 of 3) K14802 - phospholipid-transporting ATPase [EC:3.6.3.1] (DRS2, ATP8A); ATPase, aminophospholipid transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGGTCTTCATCTCCTCGTTAGGCGCGTAGTTGGGGTCCAGGCGCTC |
| Internal bar code: | ATGTGGTTAGTAGTGGTCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3182 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGTACGAGGTGAGGCCGA |
| Suggested primer 2: | TGGGCTAAGGTCATCCCGTA |