| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051357 |
| Chromosome: | chromosome 12 |
| Location: | 3696528 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGGAGCCGCGCTGGGGCACCATCCTGTCTGAGAGCATGGGCGAGGGC |
| Internal bar code: | GGAGATTGCTGATTGTGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 959 |
| LEAP-Seq percent confirming: | 47.3684 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTACTCACGCCTGCAAAG |
| Suggested primer 2: | GCAACGACAAAGAAGGCGTT |