Insertion junction: CLIP2.051365_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.051365
Chromosome:chromosome 12
Location:700268
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g492850 POC19 Proteome of centriole protein 19; (1 of 1) PF10595 - Uncharacterised protein family UPF0564 (UPF0564) 5'UTR
Cre12.g492900 (1 of 3) PF01661 - Macro domain (Macro) outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCCTCTTCTCGTCCGATTGTCCTCTCCTCGTATACAATTCGTTACATT
Internal bar code:TGGGATGAGAATGTGAAGTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:7000
LEAP-Seq percent confirming:96.7213
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCCAAATCCGCACTGTG
Suggested primer 2:GTCCTTGGACTTCTCACGCA