| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051391 |
| Chromosome: | chromosome 12 |
| Location: | 2609209 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506350 | HFO18 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | CDS |
| Cre12.g506400 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCTGGAGAACGTCATCCGTGACTCGGTCACCTACACCGAGCACGCTC |
| Internal bar code: | AGTTCGCGTCCGAGTGAATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2255 |
| LEAP-Seq percent confirming: | 43.75 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGAGGCTTCTGCTATGCG |
| Suggested primer 2: | AAATCGTGGGCGGTTGTTTG |