| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051403 |
| Chromosome: | chromosome 7 |
| Location: | 2602705 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330100 | GTR25,STT3B | (1 of 1) PTHR13872//PTHR13872:SF23 - 60S RIBOSOMAL PROTEIN L35 // DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B; Staurosporin and temperature sensitive 3-like B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTACACGGGCGCCGGACACCCAAGCGCAACAACGCAAGGGCGCCTGCA |
| Internal bar code: | GAAATAGGTCATGTTTCGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3698 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCGACCTGACACTATCTC |
| Suggested primer 2: | AACTTCTTGGCGTAGGTGGG |