Insertion junction: CLIP2.051418_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.051418
Chromosome:chromosome 6
Location:2467499
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269000 (1 of 1) PF09493 - Tryptophan-rich protein (DUF2389) (DUF2389) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGCGCGAGCGACTTCACAAGTGGTGCGTGAGCGGCGGGTGTTATGGTG
Internal bar code:CAGTGTTCATCGGGTTATGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3013
LEAP-Seq percent confirming:88.3721
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGCTGTGTAAAGCAACAA
Suggested primer 2:CTGTCTGGCATCACACCCAT