| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051422 |
| Chromosome: | chromosome 12 |
| Location: | 9874329 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543902 | (1 of 1) 2.7.1.16 - Ribulokinase / L-ribulokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTGGGAATTAGGCGTGCTGGGTAAACGTTGTTTAGCGCGCCCAGGGT |
| Internal bar code: | GATATAGTACGTTCTCCGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1197 |
| LEAP-Seq percent confirming: | 53.8462 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCGTGCCTTCCTCACTT |
| Suggested primer 2: | CGCGACACATTTAAGGCTCG |