Insertion junction: CLIP2.051436_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.051436
Chromosome:chromosome 2
Location:6222157
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g386850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
Internal bar code:GGTGGCGGCGCACAATCTCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1793
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CAACTTTGACCAGGTGTGCG
Suggested primer 2:GCCCACTTTCCAAACAGCTG