| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051452 |
| Chromosome: | chromosome 12 |
| Location: | 3236212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499650 | (1 of 2) PF06127 - Protein of unknown function (DUF962) (DUF962) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCAGCAGCAGCATAGTGGTAGCGGAGCAGCAGCAGCGGCAGCAGCAG |
| Internal bar code: | TTATATTGGTCACCCACGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3143 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACACCTCAACGCAGAGTG |
| Suggested primer 2: | ACGTGTCACACAGATCCACC |