Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.051454 |
Chromosome: | chromosome 11 |
Location: | 1997893 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467768 | (1 of 1) K10277 - lysine-specific demethylase 8 [EC:1.14.11.27] (KDM8, JMJD5) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACACACACACGCACAGCACACACAGCCGTCTCTCACGTCGTCGCCTA |
Internal bar code: | AGTGCGTCTTACATAAGGGGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2305 |
LEAP-Seq percent confirming: | 93.9394 |
LEAP-Seq n confirming: | 31 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTCTCACCCTTCCGGCAAG |
Suggested primer 2: | GCTTCCCTACTTGGTCACCC |