| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051456 |
| Chromosome: | chromosome 12 |
| Location: | 1259184 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489850 | STPK10,STK10 | Serine/threonine protein kinase; (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACCAGGGAACGCGCTTCCCCTGGTCACACTGCTGGTTGAACCGCCC |
| Internal bar code: | ACTGATTATGGGCTTCGTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4557 |
| LEAP-Seq percent confirming: | 97.2603 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGATAGGCGAGCTGAGG |
| Suggested primer 2: | CCCCTACCCATTGTCCACAC |