| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051465 |
| Chromosome: | chromosome 7 |
| Location: | 3319595 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334750 | PPP30 | (1 of 3) PTHR12320//PTHR12320:SF15 - PROTEIN PHOSPHATASE 2C // SUBFAMILY NOT NAMED; Phosphoprotein phosphatase 2C-related | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGACGTTGTTTATCGCCGCCTTACAAAAAGCTAGTTTAGGCGCCTTCC |
| Internal bar code: | GCGTTGTTGCCAATCGTGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4389 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCAGTAACTCCGTGCACC |
| Suggested primer 2: | GAGCTGGTAGGGCATGTTGT |