| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051466 |
| Chromosome: | chromosome 12 |
| Location: | 2029321 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510500 | 3'UTR | ||
| Cre12.g510550 | (1 of 2) PTHR12197//PTHR12197:SF145 - SET AND MYND DOMAIN CONTAINING // SET DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTCAGCATGGTTGCGGATACTTCAAATAGCTAGTATGATATAGTTAG |
| Internal bar code: | GTGATCGCTTAGTTGTACCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCATGACACCACTTGCTA |
| Suggested primer 2: | CGATGTCAGTAGTCTCGCCC |