Insertion junction: CLIP2.051468_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.051468
Chromosome:chromosome 6
Location:3881916
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278186 (1 of 1) IPR000157//IPR011251//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Luciferase-like domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTGCGCTGCTGGCGCAGATCATCTCCAACTCGGCGGCCTACCGCGAG
Internal bar code:CTAACTAGTTCTTATAGTTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:495
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGGTTGTTGGCGGACAC
Suggested primer 2:ACGACGAATCCACCAAGCTT