Insertion junction: CLIP2.051475_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.051475
Chromosome:chromosome 2
Location:6437430
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388450 ELG29 Exostosin-like glycosyltransferase 29; (1 of 2) PTHR31389:SF4 - PROTEIN F32B4.1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGGTGGCGGTGGGGAGGAGGAGTGTGGGCGCGGCGCGGCGCGACCGG
Internal bar code:TTCAGTTAGTATCGGTCTTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3323
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCACCAAAATTCGACCC
Suggested primer 2:GGTTCCCCGTTAAAGCAGGA