| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051477 |
| Chromosome: | chromosome 13 |
| Location: | 4134509 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591150 | HTA38,HTA22 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGTAGGAGGCTCTTGTGACCTTCCGCTATGGAGCAGAGTGTATGGC |
| Internal bar code: | ATGCACAGGTCCGGCCCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2667 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGGCAGCCTTCTTAGC |
| Suggested primer 2: | CCTGTTGACCCCCTTCTTCC |