Insertion junction: CLIP2.051479_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.051479
Chromosome:plastome
Location:31559
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802280 psaA_exon1,ChreCp019,2717000 (1 of 15) 1.97.1.12 - Photosystem I; photosystem I P700 apoprotein A1 transpliced exon 1 of 3 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTAAATTAGACAAATAGTTTTTTTTACATCATATGTTATATTATACCA
Internal bar code:TCAGCTGAGATGCACTCTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:45
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTGAAGCTCGTCTACGTG
Suggested primer 2:CCGCAACATTTTCCTTGGCA