| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.051486 |
| Chromosome: | chromosome 12 |
| Location: | 2721775 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504800 | HTR16 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGTCCGCGAGATTGCCCAGGACTTCAAGACCGACCTGCGCTTCCAGA |
| Internal bar code: | TGGTTTGGCATGATTAAGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1024 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGAGTGTTGGGTTTG |
| Suggested primer 2: | CTCCGATCGTTCTCAGCCTC |