| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.051488 |
| Chromosome: | chromosome 1 |
| Location: | 6304591 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044800 | DGAT1,PFL1 | (1 of 1) 2.3.1.54 - Formate C-acetyltransferase / Pyruvate formate-lyase; Pyruvate-formate lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAACTACACCGCTTACGCCGGCAACTCGTCCTTCCTGGCTGGCCCCA |
| Internal bar code: | AGATCTTACAGAGACTGCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1190 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGCCGTACGACTCCAG |
| Suggested primer 2: | CCCAGTCTCGTTGCACTCAT |