Insertion junction: CLIP2.051500_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.051500
Chromosome:chromosome 13
Location:1630684
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g573550 ANK26 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGAAACATACACCGACAATTGCTCAGCGCCTCCACCCACTCTGACCCA
Internal bar code:CATCAGCCGGTAACGTTCAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:244
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGAGTCAAGAGGGAGGA
Suggested primer 2:CACCCTACCTCACAACACCC