Insertion junction: CLIP2.051501_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.051501
Chromosome:chromosome 17
Location:5480633
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g739150 CTPA5,TSP5 (1 of 8) 3.4.21.102 - C-terminal processing peptidase / Tsp protease; putative C-terminal peptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACAGTACCTAGCGTTTTCCCCCGCCAGCCCATGGTGCTGACACC
Internal bar code:GTCCATGATATGTGCTTTAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:931
LEAP-Seq percent confirming:40.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCAGGCACTCCTACAATT
Suggested primer 2:CAATAGCATCGCCTGTGCAC