| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051502 |
| Chromosome: | chromosome 10 |
| Location: | 1324625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427453 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACCATCGCTTACCGCCAGGTGGGATAGCTCGAAGTCACAGCACCGCG |
| Internal bar code: | AAGTACTGGGTTCGACTTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5344 |
| LEAP-Seq percent confirming: | 84.507 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTGCGCATCTTCATCA |
| Suggested primer 2: | GCTCTGTCGAACTGCTCAGT |