| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051510 |
| Chromosome: | chromosome 2 |
| Location: | 6892316 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388763 | (1 of 1) PTHR14226//PTHR14226:SF32 - NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGCTTGTCGGGATTGGCCAAAACGTGCCCCAGTCTGCAAGCAGCTCA |
| Internal bar code: | AAGCTTTACGCAGACTAGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5672 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAACGCAATGTCTCCAGCA |
| Suggested primer 2: | GCGTCAGGTCCTCGTGATAG |